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Construction of Cell Culture Model of A. Baumannii Infection

In recent years, cell culture models of A. baumannii infection have emerged as a valuable tool for studying the pathogenesis of this bacterium and evaluating potential therapies. Ace Infectious specializes in the development of cell culture models of A. baumannii infection. Our team of experts has extensive experience in cell culture and infectious disease research, and we use state-of-the-art techniques to construct and optimize models that are suitable for a wide range of applications.

Cell Culture Model of A. Baumannii Infection

A. baumannii is a bacterial pathogen that has become a significant problem in healthcare settings worldwide due to its ability to develop resistance to multiple antibiotics. These models involve the use of cultured cells that are infected with A. baumannii, allowing researchers to investigate the cellular and molecular mechanisms of infection and test the efficacy of new drugs and other interventions.

Alveolar macrophages infected by A. baumannii.Fig. 1 Alveolar macrophages infected by A. baumannii. (Davies A H, et al., 2011)

Suitable Cell Lines for A. Baumannii Infection

One of the key challenges in developing a cell culture model of A. baumannii infection is choosing an appropriate cell line for the study. Ideally, the cell line should be permissive to A. baumannii infection, meaning that the bacteria can enter and replicate within the cells. Additionally, the cell line should be relevant to the pathophysiology of A. baumannii infections and exhibit similar responses to infection as seen in vivo.

We provide several cell lines can be used in the studies of A. baumannii infection.

  • Human lung epithelial cells (A549)
  • Human bronchial epithelial cells (16HBE)
  • Human respiratory epithelial cells (Calu-3)
  • Human macrophage-like cells (THP-1)

The choice of cell line will depend on the specific research question being addressed.

Our Cell Model Development Service

We offer a comprehensive cell model development service for A. baumannii infection. Our service includes the following steps:

  • Cell line selection - We will work with you to identify the most suitable cell line for your research question, taking into account factors such as the availability of the cell line, its permissiveness to A. baumannii infection, and its relevance to the pathophysiology of A. baumannii infections.
  • Optimization of infection conditions - We will optimize the infection conditions to achieve the highest level of infection while minimizing cellular toxicity. This may involve varying the bacterial inoculum, the length of infection, and the use of antibiotics to control bacterial growth.
  • Validation of the model - We will validate the cell model by assessing the expression of key genes and proteins involved in the host response to A. baumannii infection, as well as evaluating the response of the infected cells to known anti-infective agents.
  • Customization of the model - We can customize the cell model to meet your specific research needs, including the use of reporter genes to monitor infection, the incorporation of co-culture systems to study interactions between different cell types, and the use of patient-derived cells to study the host response to A. baumannii infection.

Application of Cell Culture Model of A. Baumannii Infection

Our cell culture model of A. baumannii infection has broad applications in the study of the pathogenesis of A. baumannii infections and the development of new therapeutics.

  • Screening of anti-infective agents
  • Investigation of bacterial virulence factors
  • Understanding the host immune response

Please do not hesitate to contact us to customize your desired cell model. We look forward to your inquiry.

References

  1. Qiu H, et al. Role of macrophages in early host resistance to respiratory Acinetobacter baumannii infection. PloS one, 2012, 7(6): e40019.
  2. McConnell M J, et al. Acinetobacter baumannii: human infections, factors contributing to pathogenesis and animal models. FEMS microbiology reviews, 2013, 37(2), 130-155.
All of our services are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
Get in touch with our team immediately.