Construction of Phage Libraries Against Superbugs
In order to develop phage therapies targeting specific bacteria, it is crucial to collect a large number of bacterial viruses to become phage libraries. Ace Therapeutics offers phage library construction services for superbugs, including specific projects such as collecting phages, virus examination, and matching phages to bacteria.
Hunting for Phage Against Superbugs
Finding possible matching phages based on the target bacteria is a critical step in phage therapy development. This is possible by collecting bacteriophages from farms, sewage, hospital waste, and other sources of human relevance. Collecting a large number of different phages from the environment could become a library of phages for drug development.
In order to make phage therapy a reality, having large phage libraries as reaction systems in research can accelerate drug development.
Construction Services of Phage Libraries Against Superbugs
Phage therapies require well-characterized phage libraries. Here, we provide services for the construction of phage libraries of different target species against superbugs including, but not limited to,
- Escherichia coli
- Helicobacter pylori
- Pseudomonas aeruginosa
- Staphylococcus aureus (MRSA)
- Enterococci bacteria
- Acinetobacter baumannii
- Campylobacter spp.
- Neisseria gonorrhoeae
We help researchers generate and store well-characterized phage libraries, which can be used to test and screen out bacteriophages that are sensitive. In subsequent design development, it is selected for use in monophage therapy or to construct phage cocktails.
- Phage isolation. We isolate phages from various samples by phage spot assay. Using the target strains as hosts, phages will be spread on preparatory plates and collected for storage.
- Phage characterization. We characterize and present basic features of phages, sequence analysis, growth characteristics, and information related to hosting range and virulence against many contemporary clinical isolates.
- Library construction. Our libraries contain a variety of parameters including information on phage origin, morphology, growth characteristics, DNA sequences, and host range. Integration of all parameters can be used to select phages for the treatment of superbug infections, including new phage isolates and commonly used phages.
In conclusion, the construction of phage libraries targeting superbugs has shown promising results in drug development. To combat superbug infections, our service aims to identify and test effective phages from a variety of host bacteria to develop targeted phage therapies.
Our Advantages
- DNA sequence analysis and morphological information from our phage library provide important information for building phage cocktails.
- Data including virulence, antibiotic resistance, adsorption constants, incubation period, and outbreak size are parameters that influence the success of phage treatment.
- Our library information can help scientists predict the outcome of in vivo treatments.
Knowledge of all these parameters facilitates the design and development of phage therapies for superbug infections. All our phage stocks can be quickly put into use and the constructed phage libraries can be quickly used for in vivo studies in animal models.
Phages are likely to be an essential complement to drug-resistant bacterial treatment, and their role is expected to be more elucidated. Contact us if you need phage library construction for specific host bacteria.
Reference
- Gibson S B, et al. Constructing and Characterizing Bacteriophage Libraries for Phage Therapy of Human Infections. Front Microbiol, 2019, 10: 2537.